[Buckle et al (2005) Nature Methods.
2
,3]
[Chow et al (2005) Protein Expr Purif.
46
, 166-171]
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Browse Database
BLAST Sequence
Protein Folding Database
Statistics
Version:
4.0
Last Updated:
2009-09-19
Refolding entries:
1157
Proteins:
759
Families:
260
Organisms:
327
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Graphical summary
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Search Results
Found
1081
results. Click on column header to sort. Click on
to expand or
to view refolding data.
Cluster/Browse by:
[Structural Class]
[Family]
[Organism]
[Refolding Method]
Expand All
Collapse All
Refolding Method
(38)
Absorbtion/Centrifugation
(2)
Protein
Hen egg- white lysozyme
(1)
Organism
Molecularity
MW (Da)
Solubility
Refolding Method
Redox Agent
pH
T(°C)
Chicken (Gallus gallus)
Unknown
14471.1
Absorbtion/Centrifugation
GSH/GSSG
8.5
25
Replication Protein A 70
(1)
Organism
Molecularity
MW (Da)
Solubility
Refolding Method
Redox Agent
pH
T(°C)
Escherichia coli
Unknown
68138.2
insoluble
Absorbtion/Centrifugation
Beta-mercaptoethanol
7.5
4
Additive-introduced stepwise dialysis
(1)
Protein
PSC33 (Ara h 6)
(1)
Organism
Molecularity
MW (Da)
Solubility
Refolding Method
Redox Agent
pH
T(°C)
Arachis Hypogaea
Monomer
14845.6
insoluble
Additive-introduced stepwise dialysis
GSH/GSSG
8
4
Centrifugal filtration
(1)
Protein
Uncoupling protein 2
(1)
Organism
Molecularity
MW (Da)
Solubility
Refolding Method
Redox Agent
pH
T(°C)
Human
Dimer
33229.4
insoluble
Centrifugal filtration
None
7.3
4
Chaperone-assisted refolding
(5)
Protein
Cyclophilin, Variant A
(1)
Organism
Molecularity
MW (Da)
Solubility
Refolding Method
Redox Agent
pH
T(°C)
Human
Monomer
17881.3
Chaperone-assisted refolding
None
7.8
25
Interferon gamma
(1)
Organism
Molecularity
MW (Da)
Solubility
Refolding Method
Redox Agent
pH
T(°C)
Human
Dimer
17145.6
insoluble
Chaperone-assisted refolding
None
7
25
Lipase chaperone
(1)
Organism
Molecularity
MW (Da)
Solubility
Refolding Method
Redox Agent
pH
T(°C)
Burkholderia cepacia (Pseudomonas cepacia)
Unknown
36321.7
partial
Chaperone-assisted refolding
None
7.2
4
Outer membrane protein A
(1)
Organism
Molecularity
MW (Da)
Solubility
Refolding Method
Redox Agent
pH
T(°C)
Escherichia coli
Unknown
35172.3
Chaperone-assisted refolding
None
7.5
25
Prochymosin
(1)
Organism
Molecularity
MW (Da)
Solubility
Refolding Method
Redox Agent
pH
T(°C)
Bovine
Monomer
42179.7
insoluble
Chaperone-assisted refolding
None
10.7
25
Chaperone-assisted/reversed micelles
(1)
Protein
Ribonuclease A
(1)
Organism
Molecularity
MW (Da)
Solubility
Refolding Method
Redox Agent
pH
T(°C)
Bovine
Monomer
13690.3
Chaperone-assisted/reversed micelles
GSH/GSSG
8.5
30
Chitin Column Chromatography
(1)
Protein
Lipase
(1)
Organism
Molecularity
MW (Da)
Solubility
Refolding Method
Redox Agent
pH
T(°C)
Burkholderia cepacia KWI-56
Monomer
33174.1
insoluble
Chitin Column Chromatography
None
8
20
Column refolding- Immobilized Metal affinity chromatography (IMAC)
(2)
Protein
Extracellular Superoxide Dismutase
(1)
Organism
Molecularity
MW (Da)
Solubility
Refolding Method
Redox Agent
pH
T(°C)
Human
Tetramer
24132.8
insoluble
Column refolding- Immobilized Metal affinity chromatography (IMAC)
GSH/GSSG
8
22
Serine protease/NS3 protein/helicase NS3
(2)
Organism
Molecularity
MW (Da)
Solubility
Refolding Method
Redox Agent
pH
T(°C)
Hepatitis C virus genotype 1b
Unknown
67141.7
insoluble
Column refolding- Immobilized Metal affinity chromatography (IMAC)
Beta-mercaptoethanol
8
25
Hepatitis C virus genotype 1b
Unknown
67141.7
insoluble
Column refolding- Immobilized Metal affinity chromatography (IMAC)
Beta-mercaptoethanol
8
25
Column refolding-Expanded bed adsorption chromatography (EBA)
(3)
Protein
Apolipoprotein(a)(lipoprotein kringle)
(1)
Organism
Molecularity
MW (Da)
Solubility
Refolding Method
Redox Agent
pH
T(°C)
Human
Unknown
36696.7
insoluble
Column refolding-Expanded bed adsorption chromatography (EBA)
None
9
25
Epidermal Growth Factor
(1)
Organism
Molecularity
MW (Da)
Solubility
Refolding Method
Redox Agent
pH
T(°C)
Human
Monomer
6222
insoluble
Column refolding-Expanded bed adsorption chromatography (EBA)
None
6.5
25
Interferon gamma
(1)
Organism
Molecularity
MW (Da)
Solubility
Refolding Method
Redox Agent
pH
T(°C)
Human
Unknown
16177.5
insoluble
Column refolding-Expanded bed adsorption chromatography (EBA)
None
7.5
0
Column refolding: affinity immobilization
(4)
Protein
Cobrotoxin
(1)
Organism
Molecularity
MW (Da)
Solubility
Refolding Method
Redox Agent
pH
T(°C)
Naja atra (Chinese cobra)
Unknown
6956.6
insoluble
Column refolding: affinity immobilization
GSH/GSSG
8
4
Extracellular zinc metalloprotease
(1)
Organism
Molecularity
MW (Da)
Solubility
Refolding Method
Redox Agent
pH
T(°C)
Burkholderia cepacia KWI-56
Unknown
57347.4
partial
Column refolding: affinity immobilization
None
8
4
Hepatitis C virus core protein
(2)
Organism
Molecularity
MW (Da)
Solubility
Refolding Method
Redox Agent
pH
T(°C)
Hepatitis C virus
Dimer
19637.4
insoluble
Column refolding: affinity immobilization
Beta-mercaptoethanol
7.5
20
Hepatitis C virus
Dimer
14399.4
insoluble
Column refolding: affinity immobilization
Beta-mercaptoethanol
7.5
20
myelin oligodendrocyte glycoprotein
(1)
Organism
Molecularity
MW (Da)
Solubility
Refolding Method
Redox Agent
pH
T(°C)
Mouse
Dimer
14591.4
insoluble
Column refolding: affinity immobilization
Beta-mercaptoethanol/GSH-GSSG
8
25
Column refolding: Cobalt-chelating chromatography
(1)
Protein
Voltage-dependent Anion Channel 1
(1)
Organism
Molecularity
MW (Da)
Solubility
Refolding Method
Redox Agent
pH
T(°C)
Human
Monomer
31895.8
insoluble
Column refolding: Cobalt-chelating chromatography
None
7.9
4
Column refolding: hydrophobic interaction chromatography
(7)
Protein
Alpha-amylase
(1)
Organism
Molecularity
MW (Da)
Solubility
Refolding Method
Redox Agent
pH
T(°C)
Pyrococcus woesei
Monomer
50184
insoluble
Column refolding: hydrophobic interaction chromatography
None
6
25
Elongation factor G
(2)
Organism
Molecularity
MW (Da)
Solubility
Refolding Method
Redox Agent
pH
T(°C)
Staphylococcus aureus
Unknown
76481.2
partial
Column refolding: hydrophobic interaction chromatography
Beta-mercaptoethanol
8.6
0
Staphylococcus aureus
Unknown
76481.2
partial
Column refolding: hydrophobic interaction chromatography
GSH/GSSG
8.6
0
HIV-1 Rev binding protein-like
(1)
Organism
Molecularity
MW (Da)
Solubility
Refolding Method
Redox Agent
pH
T(°C)
Human
Unknown
48963.1
insoluble
Column refolding: hydrophobic interaction chromatography
None
0
0
Interferon gamma
(5)
Organism
Molecularity
MW (Da)
Solubility
Refolding Method
Redox Agent
pH
T(°C)
Human
Dimer
16177.5
insoluble
Column refolding: hydrophobic interaction chromatography
None
7
25
Human
Dimer
16177.5
insoluble
Column refolding: hydrophobic interaction chromatography
None
7
25
Human
Dimer
16177.5
insoluble
Column refolding: hydrophobic interaction chromatography
None
7
25
Human
Dimer
16177.5
insoluble
Column refolding: hydrophobic interaction chromatography
None
7
25
Human
Dimer
16177.5
insoluble
Column refolding: hydrophobic interaction chromatography
None
7
25
Lysozyme
(3)
Organism
Molecularity
MW (Da)
Solubility
Refolding Method
Redox Agent
pH
T(°C)
Chicken (Gallus gallus)
Unknown
4953
Column refolding: hydrophobic interaction chromatography
GSH/GSSG
8.5
0
Chicken (Gallus gallus)
Unknown
4953
Column refolding: hydrophobic interaction chromatography
Beta-mercaptoethanol
8.5
0
Chicken (Gallus gallus)
Unknown
4953
Column refolding: hydrophobic interaction chromatography
Beta-mercaptoethanol
8.5
0
Prion
(1)
Organism
Molecularity
MW (Da)
Solubility
Refolding Method
Redox Agent
pH
T(°C)
Bovine
Monomer
22747
insoluble
Column refolding: hydrophobic interaction chromatography
None
7
25
Proinsulin
(1)
Organism
Molecularity
MW (Da)
Solubility
Refolding Method
Redox Agent
pH
T(°C)
Human
Monomer
9394.7
insoluble
Column refolding: hydrophobic interaction chromatography
None
7.5
25
column refolding: ion-exchange chromatography
(7)
Protein
Apolipoprotein(a)(lipoprotein kringle)
(1)
Organism
Molecularity
MW (Da)
Solubility
Refolding Method
Redox Agent
pH
T(°C)
Human
Unknown
36696.7
insoluble
column refolding: ion-exchange chromatography
None
9
25
Bovine serum albumin
(1)
Organism
Molecularity
MW (Da)
Solubility
Refolding Method
Redox Agent
pH
T(°C)
Bovine
Monomer
66433
column refolding: ion-exchange chromatography
GSH/GSSG
8.5
25
Cell division control protein 42 homolog
(3)
Organism
Molecularity
MW (Da)
Solubility
Refolding Method
Redox Agent
pH
T(°C)
Human
Monomer
24056.8
not stated
column refolding: ion-exchange chromatography
None
8
4
Human
Monomer
24056.8
not stated
column refolding: ion-exchange chromatography
None
8
4
Human
Monomer
24056.8
not stated
column refolding: ion-exchange chromatography
None
8
25
Enhanced Green Fluorescent Protein
(1)
Organism
Molecularity
MW (Da)
Solubility
Refolding Method
Redox Agent
pH
T(°C)
Aqueorea victoria
Monomer
29014.7
insoluble
column refolding: ion-exchange chromatography
None
9
20
Hahellin
(1)
Organism
Molecularity
MW (Da)
Solubility
Refolding Method
Redox Agent
pH
T(°C)
Hahella chejuensis
Monomer
49769.5
insoluble
column refolding: ion-exchange chromatography
None
9.5
25
Human Granulocyte colony-stimulating factor
(1)
Organism
Molecularity
MW (Da)
Solubility
Refolding Method
Redox Agent
pH
T(°C)
Human
Unknown
19058.1
insoluble
column refolding: ion-exchange chromatography
GSH/GSSG
8
25
Serine protease/NS3 protein/helicase NS3
(2)
Organism
Molecularity
MW (Da)
Solubility
Refolding Method
Redox Agent
pH
T(°C)
Hepatitis C virus genotype 1b
Unknown
67141.7
insoluble
column refolding: ion-exchange chromatography
Beta-mercaptoethanol
9
25
Hepatitis C virus genotype 1b
Unknown
67141.7
insoluble
column refolding: ion-exchange chromatography
Beta-mercaptoethanol
9
25
Column refolding: Nickel-chelating chromatography
(46)
Protein
30K protein
(1)
Organism
Molecularity
MW (Da)
Solubility
Refolding Method
Redox Agent
pH
T(°C)
Bombyx mori
Unknown
29666.8
insoluble
Column refolding: Nickel-chelating chromatography
None
0
0
Aequorin
(1)
Organism
Molecularity
MW (Da)
Solubility
Refolding Method
Redox Agent
pH
T(°C)
Aqueorea victoria
Monomer
22641.4
insoluble
Column refolding: Nickel-chelating chromatography
Beta-mercaptoethanol
7.8
4
Alpha-2-macroglobulin
(1)
Organism
Molecularity
MW (Da)
Solubility
Refolding Method
Redox Agent
pH
T(°C)
Human
Unknown
16924.5
insoluble
Column refolding: Nickel-chelating chromatography
None
8
25
Alpha-tocopherol transfer protein
(1)
Organism
Molecularity
MW (Da)
Solubility
Refolding Method
Redox Agent
pH
T(°C)
Human
Monomer
36124.5
partial
Column refolding: Nickel-chelating chromatography
None
7.6
14
APG8a (At4g21980) autophagy protein
(1)
Organism
Molecularity
MW (Da)
Solubility
Refolding Method
Redox Agent
pH
T(°C)
Arabidopsis thaliana
Monomer
17184.7
partial
Column refolding: Nickel-chelating chromatography
None
7
25
Apical membrane antigen 1
(1)
Organism
Molecularity
MW (Da)
Solubility
Refolding Method
Redox Agent
pH
T(°C)
Plasmodium chabaudi
Monomer
73876.1
insoluble
Column refolding: Nickel-chelating chromatography
None
8.3
25
Botulinum neurotoxin type A
(1)
Organism
Molecularity
MW (Da)
Solubility
Refolding Method
Redox Agent
pH
T(°C)
Clostridium botulinum
Monomer
27882.5
insoluble
Column refolding: Nickel-chelating chromatography
Beta-mercaptoethanol
8
25
CC Chemokine Lymphotactin
(1)
Organism
Molecularity
MW (Da)
Solubility
Refolding Method
Redox Agent
pH
T(°C)
Human
Unknown
38507.5
insoluble
Column refolding: Nickel-chelating chromatography
GSH/GSSG
8
25
CC Chemokine RANTES
(1)
Organism
Molecularity
MW (Da)
Solubility
Refolding Method
Redox Agent
pH
T(°C)
Human
Dimer
9989.7
insoluble
Column refolding: Nickel-chelating chromatography
GSH/GSSG
8
25
Cholera enterotoxin subunit A [Precursor]
(1)
Organism
Molecularity
MW (Da)
Solubility
Refolding Method
Redox Agent
pH
T(°C)
Vibrio cholerae
Unknown
27194
insoluble
Column refolding: Nickel-chelating chromatography
None
7.5
1
Circumsporozoite protein
(1)
Organism
Molecularity
MW (Da)
Solubility
Refolding Method
Redox Agent
pH
T(°C)
Plasmodium falciparum
Unknown
42596.3
insoluble
Column refolding: Nickel-chelating chromatography
None
0
25
CX3C chemokine fractalkine
(1)
Organism
Molecularity
MW (Da)
Solubility
Refolding Method
Redox Agent
pH
T(°C)
Human
Unknown
39593.4
insoluble
Column refolding: Nickel-chelating chromatography
GSH/GSSG
8
25
Dehydrodolichyl diphosphate synthase
(1)
Organism
Molecularity
MW (Da)
Solubility
Refolding Method
Redox Agent
pH
T(°C)
Yeast (Saccharomyces cerevisiae)
Monomer
32693.6
partial
Column refolding: Nickel-chelating chromatography
Beta-mercaptoethanol
7.5
25
DevS Histidine Protein Kinase
(1)
Organism
Molecularity
MW (Da)
Solubility
Refolding Method
Redox Agent
pH
T(°C)
Mycobacterium tuberculosis
Monomer
62228.6
insoluble
Column refolding: Nickel-chelating chromatography
GSH/GSSG
8
16
Exopolyphosphatase
(1)
Organism
Molecularity
MW (Da)
Solubility
Refolding Method
Redox Agent
pH
T(°C)
Trypanosoma brucei
Unknown
43817.2
insoluble
Column refolding: Nickel-chelating chromatography
None
7.9
25
Extracellular domain of myelin oligodendrocyte glycoprotein
(1)
Organism
Molecularity
MW (Da)
Solubility
Refolding Method
Redox Agent
pH
T(°C)
Mouse
Unknown
28296
Column refolding: Nickel-chelating chromatography
GSSG/beta mercaptoethanol
8
4
Fel d 1
(1)
Organism
Molecularity
MW (Da)
Solubility
Refolding Method
Redox Agent
pH
T(°C)
Felis domesticus (cat)
Heterodimer
18795.6
insoluble
Column refolding: Nickel-chelating chromatography
None
8
25
Fibroblast growth factor receptor
(1)
Organism
Molecularity
MW (Da)
Solubility
Refolding Method
Redox Agent
pH
T(°C)
Human
Monomer
15087
insoluble
Column refolding: Nickel-chelating chromatography
None
7.5
25
Flotillin-1
(1)
Organism
Molecularity
MW (Da)
Solubility
Refolding Method
Redox Agent
pH
T(°C)
Human
Unknown
47355.3
insoluble
Column refolding: Nickel-chelating chromatography
None
8
25
Glutamyl-tRNA reductase
(1)
Organism
Molecularity
MW (Da)
Solubility
Refolding Method
Redox Agent
pH
T(°C)
Escherichia coli
Tetramer
48873.6
partial
Column refolding: Nickel-chelating chromatography
Beta-mercaptoethanol
8
25
Glyceraldehyde-3-phosphate dehydrogenase
(1)
Organism
Molecularity
MW (Da)
Solubility
Refolding Method
Redox Agent
pH
T(°C)
Paracoccidioides brasiliensis
Unknown
36470.4
insoluble
Column refolding: Nickel-chelating chromatography
None
6
25
Granzyme B
(2)
Organism
Molecularity
MW (Da)
Solubility
Refolding Method
Redox Agent
pH
T(°C)
Human
Monomer
27322.4
insoluble
Column refolding: Nickel-chelating chromatography
GSH/GSSG
8
10
Human
Monomer
27378.5
insoluble
Column refolding: Nickel-chelating chromatography
GSH/GSSG
8
10
Indole 3-glycerol phosphate synthase
(1)
Organism
Molecularity
MW (Da)
Solubility
Refolding Method
Redox Agent
pH
T(°C)
Escherichia coli
Monomer
23015.4
Column refolding: Nickel-chelating chromatography
None
7.8
25
Lectin
(1)
Organism
Molecularity
MW (Da)
Solubility
Refolding Method
Redox Agent
pH
T(°C)
Common snowdrop (Galanthus nivalis)
Dimer
16917.4
soluble
Column refolding: Nickel-chelating chromatography
None
7.9
25
Leukocyte cell-derived chemotaxin 2
(1)
Organism
Molecularity
MW (Da)
Solubility
Refolding Method
Redox Agent
pH
T(°C)
Human
Unknown
14572.7
insoluble
Column refolding: Nickel-chelating chromatography
GSH/GSSG
7.9
4
Merozoite Surface Protein-1
(1)
Organism
Molecularity
MW (Da)
Solubility
Refolding Method
Redox Agent
pH
T(°C)
Plasmodium chabaudi
Monomer
42826.4
insoluble
Column refolding: Nickel-chelating chromatography
None
8.3
25
Monoclonal anti-idiotypic Schistosoma japonicum antibody NP30 immunoglobulin lig
(1)
Organism
Molecularity
MW (Da)
Solubility
Refolding Method
Redox Agent
pH
T(°C)
Mouse
Unknown
11477.8
insoluble
Column refolding: Nickel-chelating chromatography
None
8
25
Outer membrane protein 21
(2)
Organism
Molecularity
MW (Da)
Solubility
Refolding Method
Redox Agent
pH
T(°C)
Comamonas acidovorans
Monomer
23357.7
insoluble
Column refolding: Nickel-chelating chromatography
None
7.5
25
Comamonas acidovorans
Monomer
20589.3
insoluble
Column refolding: Nickel-chelating chromatography
None
7.5
25
oxoglutarate-malate carrier
(1)
Organism
Molecularity
MW (Da)
Solubility
Refolding Method
Redox Agent
pH
T(°C)
Bovine
Unknown
34040.7
insoluble
Column refolding: Nickel-chelating chromatography
DTT
7.4
4
Polyhydroxyalkanoate synthase
(1)
Organism
Molecularity
MW (Da)
Solubility
Refolding Method
Redox Agent
pH
T(°C)
Pseudomonas aeruginosa
Monomer
67693.7
insoluble
Column refolding: Nickel-chelating chromatography
Beta-mercaptoethanol
8
25
PPE FAMILY PROTEIN
(1)
Organism
Molecularity
MW (Da)
Solubility
Refolding Method
Redox Agent
pH
T(°C)
Mycobacterium tuberculosis
Unknown
21942.8
insoluble
Column refolding: Nickel-chelating chromatography
GSH
8
25
Prion
(1)
Organism
Molecularity
MW (Da)
Solubility
Refolding Method
Redox Agent
pH
T(°C)
Human
Monomer
27661.2
insoluble
Column refolding: Nickel-chelating chromatography
None
8
25
Prohibitin
(2)
Organism
Molecularity
MW (Da)
Solubility
Refolding Method
Redox Agent
pH
T(°C)
Human
Tetramer
31366.7
insoluble
Column refolding: Nickel-chelating chromatography
None
8
37
Human
Tetramer
28272.1
insoluble
Column refolding: Nickel-chelating chromatography
None
8
37
Prostasin
(1)
Organism
Molecularity
MW (Da)
Solubility
Refolding Method
Redox Agent
pH
T(°C)
Human
Heterodimer
33337.8
insoluble
Column refolding: Nickel-chelating chromatography
GSH/GSSG
8
1
Protective antigen
(1)
Organism
Molecularity
MW (Da)
Solubility
Refolding Method
Redox Agent
pH
T(°C)
Bacillus anthracis
Unknown
85811.1
insoluble
Column refolding: Nickel-chelating chromatography
None
7.8
25
Recombinant grass pollen hybrid molecule
(1)
Organism
Molecularity
MW (Da)
Solubility
Refolding Method
Redox Agent
pH
T(°C)
Phleum pratense (Common timothy)
Unknown
59311.9
insoluble
Column refolding: Nickel-chelating chromatography
None
7.4
25
RNA Dependent RNA Polymerase
(1)
Organism
Molecularity
MW (Da)
Solubility
Refolding Method
Redox Agent
pH
T(°C)
Hepititis E Virus
Monomer
43613.3
insoluble
Column refolding: Nickel-chelating chromatography
None
7.4
25
RNA polymerase
(1)
Organism
Molecularity
MW (Da)
Solubility
Refolding Method
Redox Agent
pH
T(°C)
Escherichia coli
Monomer
25624.5
insoluble
Column refolding: Nickel-chelating chromatography
DTT
7.5
20
Serotonin N-acetyltransferase
(3)
Organism
Molecularity
MW (Da)
Solubility
Refolding Method
Redox Agent
pH
T(°C)
Human
Unknown
23344.1
insoluble
Column refolding: Nickel-chelating chromatography
None
8
25
Ovis aries (sheep/ovine)
Unknown
23076.5
insoluble
Column refolding: Nickel-chelating chromatography
None
8
25
Rat
Unknown
23141.9
insoluble
Column refolding: Nickel-chelating chromatography
None
8
25
Sj Serpin
(1)
Organism
Molecularity
MW (Da)
Solubility
Refolding Method
Redox Agent
pH
T(°C)
Schistosoma japonicum
Monomer
48600.5
insoluble
Column refolding: Nickel-chelating chromatography
Not specified
7.4
4
Stromal cell-derived factor-1a
(1)
Organism
Molecularity
MW (Da)
Solubility
Refolding Method
Redox Agent
pH
T(°C)
Human
Unknown
7610
insoluble
Column refolding: Nickel-chelating chromatography
None
8
25
T-Cell receptor D10 single chain
(1)
Organism
Molecularity
MW (Da)
Solubility
Refolding Method
Redox Agent
pH
T(°C)
Mouse
Monomer
28534.4
soluble
Column refolding: Nickel-chelating chromatography
None
8
25
Triosephosphate isomerase
(1)
Organism
Molecularity
MW (Da)
Solubility
Refolding Method
Redox Agent
pH
T(°C)
Solanum chacoense (Chaco potato)
Unknown
27039.9
insoluble
Column refolding: Nickel-chelating chromatography
None
5.9
0
tumour necrosis factor alpha single-chain variable fragment
(1)
Organism
Molecularity
MW (Da)
Solubility
Refolding Method
Redox Agent
pH
T(°C)
Escherichia coli
Monomer
33923
insoluble
Column refolding: Nickel-chelating chromatography
GSH/GSSG
8
25
Type XI collagen alpha-1 chain
(1)
Organism
Molecularity
MW (Da)
Solubility
Refolding Method
Redox Agent
pH
T(°C)
Rattus norvegicus (Rat)
Unknown
57396.3
partial
Column refolding: Nickel-chelating chromatography
None
0
0
Ykt6p SNARE
(1)
Organism
Molecularity
MW (Da)
Solubility
Refolding Method
Redox Agent
pH
T(°C)
Arabidopsis thaliana
Unknown
24838.1
insoluble
Column refolding: Nickel-chelating chromatography
Beta-mercaptoethanol
8
25
Column refolding: Oxidative refolding chromatography
(3)
Protein
CD1a
(1)
Organism
Molecularity
MW (Da)
Solubility
Refolding Method
Redox Agent
pH
T(°C)
Human
Monomer
32465.5
insoluble
Column refolding: Oxidative refolding chromatography
GSSG
8
25
CD1b
(1)
Organism
Molecularity
MW (Da)
Solubility
Refolding Method
Redox Agent
pH
T(°C)
Human
Monomer
31178.3
insoluble
Column refolding: Oxidative refolding chromatography
GSSG
8
25
Toxin 5
(1)
Organism
Molecularity
MW (Da)
Solubility
Refolding Method
Redox Agent
pH
T(°C)
Scorpion (C. noxius)
Monomer
9480.1
soluble
Column refolding: Oxidative refolding chromatography
DTT
8
25
Column refolding: protein immobilization
(1)
Protein
Subtilisin BPN
(1)
Organism
Molecularity
MW (Da)
Solubility
Refolding Method
Redox Agent
pH
T(°C)
Bacillus amyloliquefaciens
Monomer
27462.6
Column refolding: protein immobilization
None
6.5
25
Column refolding: size exclusion chromatography with partner subunits
(2)
Protein
Integration host factor alpha subunit
(1)
Organism
Molecularity
MW (Da)
Solubility
Refolding Method
Redox Agent
pH
T(°C)
Escherichia coli
Monomer
11353.9
Column refolding: size exclusion chromatography with partner subunits
None
7.4
25
Integration host factor beta subunit
(1)
Organism
Molecularity
MW (Da)
Solubility
Refolding Method
Redox Agent
pH
T(°C)
Escherichia coli
Monomer
10651.1
Column refolding: size exclusion chromatography with partner subunits
None
7.4
25
Column refolding: Size-exclusion chromatography
(37)
Protein
B lymphocyte stimulator
(1)
Organism
Molecularity
MW (Da)
Solubility
Refolding Method
Redox Agent
pH
T(°C)
Human
Monomer
17040.5
insoluble
Column refolding: Size-exclusion chromatography
GSH/GSSG
8
4
beta-lactamase
(1)
Organism
Molecularity
MW (Da)
Solubility
Refolding Method
Redox Agent
pH
T(°C)
Escherichia coli
Monomer
39551.1
insoluble
Column refolding: Size-exclusion chromatography
None
7
0
Bothropstoxin-I
(1)
Organism
Molecularity
MW (Da)
Solubility
Refolding Method
Redox Agent
pH
T(°C)
Bothrops jararacussu
Monomer
13806.1
insoluble
Column refolding: Size-exclusion chromatography
GSH/GSSG
8
25
C-phycoerythrin class I alpha chain
(1)
Organism
Molecularity
MW (Da)
Solubility
Refolding Method
Redox Agent
pH
T(°C)
Synechococcus sp. (strain WH8102)
Unknown
17817.1
insoluble
Column refolding: Size-exclusion chromatography
Beta-mercaptoethanol
7.2
23
C1-Inhibitor
(1)
Organism
Molecularity
MW (Da)
Solubility
Refolding Method
Redox Agent
pH
T(°C)
Human
Monomer
52843.4
insoluble
Column refolding: Size-exclusion chromatography
None
7.4
25
Calcium Channel Beta1b-subunit
(1)
Organism
Molecularity
MW (Da)
Solubility
Refolding Method
Redox Agent
pH
T(°C)
Human
Monomer
66629.3
insoluble
Column refolding: Size-exclusion chromatography
DTT
8
12
Carnitine/acylcarnitine carrier protein mitochondrial
(1)
Organism
Molecularity
MW (Da)
Solubility
Refolding Method
Redox Agent
pH
T(°C)
Rat
Unknown
33154
insoluble
Column refolding: Size-exclusion chromatography
None
7
0
Cystatin C
(1)
Organism
Molecularity
MW (Da)
Solubility
Refolding Method
Redox Agent
pH
T(°C)
Human
Dimer
13347.1
insoluble
Column refolding: Size-exclusion chromatography
None
7.8
25
Cysteine proteinase
(2)
Organism
Molecularity
MW (Da)
Solubility
Refolding Method
Redox Agent
pH
T(°C)
Entamoeba histolytica
Unknown
50722.9
insoluble
Column refolding: Size-exclusion chromatography
None
8
25
Entamoeba histolytica
Unknown
50722.9
partial
Column refolding: Size-exclusion chromatography
None
8
25
Delta-like1
(1)
Organism
Molecularity
MW (Da)
Solubility
Refolding Method
Redox Agent
pH
T(°C)
Human
Unknown
11229.3
insoluble
Column refolding: Size-exclusion chromatography
None
7
0
Der f 1
(1)
Organism
Molecularity
MW (Da)
Solubility
Refolding Method
Redox Agent
pH
T(°C)
Dermatophagoides farinae (dust mite)
Unknown
34644.7
insoluble
Column refolding: Size-exclusion chromatography
None
7.6
25
Eco29kI restriction endonuclease
(1)
Organism
Molecularity
MW (Da)
Solubility
Refolding Method
Redox Agent
pH
T(°C)
Escherichia coli
Monomer
24566.6
insoluble
Column refolding: Size-exclusion chromatography
Beta-mercaptoethanol
7.2
25
ETS1
(2)
Organism
Molecularity
MW (Da)
Solubility
Refolding Method
Redox Agent
pH
T(°C)
Human
Unknown
52571.2
insoluble
Column refolding: Size-exclusion chromatography
None
7.3
25
Human
Unknown
42949.8
insoluble
Column refolding: Size-exclusion chromatography
None
7.3
25
Hen egg- white lysozyme
(1)
Organism
Molecularity
MW (Da)
Solubility
Refolding Method
Redox Agent
pH
T(°C)
Chicken (Gallus gallus)
Unknown
14471.1
Column refolding: Size-exclusion chromatography
DTT
8.2
25
Human Granulocyte colony-stimulating factor
(2)
Organism
Molecularity
MW (Da)
Solubility
Refolding Method
Redox Agent
pH
T(°C)
Human
Unknown
19058.1
insoluble
Column refolding: Size-exclusion chromatography
GSH/GSSG
8
25
Human
Unknown
18987
insoluble
Column refolding: Size-exclusion chromatography
GSH/GSSG
8
25
Interleukin-6
(1)
Organism
Molecularity
MW (Da)
Solubility
Refolding Method
Redox Agent
pH
T(°C)
Human
Monomer
23718.2
insoluble
Column refolding: Size-exclusion chromatography
None
5
22
Isopenicillin N synthetase
(1)
Organism
Molecularity
MW (Da)
Solubility
Refolding Method
Redox Agent
pH
T(°C)
Flavobacterium sp.
Unknown
36465
partial
Column refolding: Size-exclusion chromatography
DTT
8
25
Lipase
(1)
Organism
Molecularity
MW (Da)
Solubility
Refolding Method
Redox Agent
pH
T(°C)
Pseudomonas fluorescens
Monomer
32427
insoluble
Column refolding: Size-exclusion chromatography
None
8
4
LMW Urokinase Plasminogen Activator
(1)
Organism
Molecularity
MW (Da)
Solubility
Refolding Method
Redox Agent
pH
T(°C)
Human
Monomer
30962.5
insoluble
Column refolding: Size-exclusion chromatography
GSH/GSSG
8.5
4
Lysozyme
(4)
Organism
Molecularity
MW (Da)
Solubility
Refolding Method
Redox Agent
pH
T(°C)
Human
Monomer
14700.7
insoluble
Column refolding: Size-exclusion chromatography
GSH/GSSG
8
25
Human
Monomer
14700.7
insoluble
Column refolding: Size-exclusion chromatography
GSH/GSSG
8
25
Human
Monomer
14700.7
insoluble
Column refolding: Size-exclusion chromatography
GSH/GSSG
6
25
Human
Monomer
14700.7
insoluble
Column refolding: Size-exclusion chromatography
GSH/GSSG
8
25
Macrophage Metalloelastase (MMP-12)
(1)
Organism
Molecularity
MW (Da)
Solubility
Refolding Method
Redox Agent
pH
T(°C)
Rat
Monomer
53738.4
insoluble
Column refolding: Size-exclusion chromatography
None
7.5
4
metalloprotease also know as Zinc metalloproteinase/disintegrin
(1)
Organism
Molecularity
MW (Da)
Solubility
Refolding Method
Redox Agent
pH
T(°C)
Agkistrodon halys brevicaudus (Korean slamosa snak
Unknown
32184.4
insoluble
Column refolding: Size-exclusion chromatography
None
7.5
0
Nuclease
(1)
Organism
Molecularity
MW (Da)
Solubility
Refolding Method
Redox Agent
pH
T(°C)
Anabaena sp.
Monomer
27400.6
insoluble
Column refolding: Size-exclusion chromatography
GSH
8.2
0
Platelet-derived growth factor
(1)
Organism
Molecularity
MW (Da)
Solubility
Refolding Method
Redox Agent
pH
T(°C)
Human
Heterodimer
32393.8
soluble
Column refolding: Size-exclusion chromatography
GSH/GSSG
7.8
25
PorB (class 3) porin
(1)
Organism
Molecularity
MW (Da)
Solubility
Refolding Method
Redox Agent
pH
T(°C)
Neisseria meningitidis
Monomer
35740.7
insoluble
Column refolding: Size-exclusion chromatography
None
8
25
Proinsulin
(2)
Organism
Molecularity
MW (Da)
Solubility
Refolding Method
Redox Agent
pH
T(°C)
Human
Monomer
11980.9
insoluble
Column refolding: Size-exclusion chromatography
Beta-mercaptoethanol
6.8
25
Felis silvestris catus (Cat)
Unknown
9385.6
insoluble
Column refolding: Size-exclusion chromatography
DTT
8.7
50
Proteasome subunit alpha type-5
(1)
Organism
Molecularity
MW (Da)
Solubility
Refolding Method
Redox Agent
pH
T(°C)
Human
Monomer
26411
insoluble
Column refolding: Size-exclusion chromatography
GSH/GSSG/DTT
7.3
25
Ribonuclease A
(1)
Organism
Molecularity
MW (Da)
Solubility
Refolding Method
Redox Agent
pH
T(°C)
Bovine
Monomer
13860.5
not stated
Column refolding: Size-exclusion chromatography
None
7.5
4
Somatotropin also known as Growth hormone
(1)
Organism
Molecularity
MW (Da)
Solubility
Refolding Method
Redox Agent
pH
T(°C)
Ovis aries (sheep/ovine)
Monomer
21787
insoluble
Column refolding: Size-exclusion chromatography
None
8
25
Staphylokinase
(1)
Organism
Molecularity
MW (Da)
Solubility
Refolding Method
Redox Agent
pH
T(°C)
Staphylococcus aureus
Dimer
15464.6
partial
Column refolding: Size-exclusion chromatography
None
7.4
24
Stromal cell-derived factor-1a
(1)
Organism
Molecularity
MW (Da)
Solubility
Refolding Method
Redox Agent
pH
T(°C)
Human
Unknown
7610
insoluble
Column refolding: Size-exclusion chromatography
GSH/GSSG
8
22
Thrombopoietin
(1)
Organism
Molecularity
MW (Da)
Solubility
Refolding Method
Redox Agent
pH
T(°C)
Human
Unknown
16614.6
insoluble
Column refolding: Size-exclusion chromatography
None
6.5
0
Thrombopoietin receptor
(1)
Organism
Molecularity
MW (Da)
Solubility
Refolding Method
Redox Agent
pH
T(°C)
Human
Unknown
57906.7
insoluble
Column refolding: Size-exclusion chromatography
DTT
7.4
25
Trafficking protein particle complex subunit 6A
(1)
Organism
Molecularity
MW (Da)
Solubility
Refolding Method
Redox Agent
pH
T(°C)
Human
Unknown
17505.4
insoluble
Column refolding: Size-exclusion chromatography
None
0
0
Transposase Tc1A
(1)
Organism
Molecularity
MW (Da)
Solubility
Refolding Method
Redox Agent
pH
T(°C)
C. elegans
Monomer
31820.4
insoluble
Column refolding: Size-exclusion chromatography
None
5.5
25
Troponin T, fast skeletal muscle isoforms
(1)
Organism
Molecularity
MW (Da)
Solubility
Refolding Method
Redox Agent
pH
T(°C)
Chicken (Gallus gallus)
Unknown
31010.7
insoluble
Column refolding: Size-exclusion chromatography
DTT
6
0
Yersiniabactin transcriptional regulator
(2)
Organism
Molecularity
MW (Da)
Solubility
Refolding Method
Redox Agent
pH
T(°C)
Yersinia enterocolitica
Monomer
37881.3
insoluble
Column refolding: Size-exclusion chromatography
Beta-mercaptoethanol
8
37
Yersinia enterocolitica
Monomer
37881.3
insoluble
Column refolding: Size-exclusion chromatography
Beta-mercaptoethanol
8
37
Detergent precipitation/sequestration
(2)
Protein
Epidermal Growth Factor
(1)
Organism
Molecularity
MW (Da)
Solubility
Refolding Method
Redox Agent
pH
T(°C)
Human
Monomer
6222
insoluble
Detergent precipitation/sequestration
Cysteine/Cystine
9.8
22
Light harvesting complex II
(1)
Organism
Molecularity
MW (Da)
Solubility
Refolding Method
Redox Agent
pH
T(°C)
Arabidopsis thaliana
Trimer
24863.2
insoluble
Detergent precipitation/sequestration
Beta-mercaptoethanol
9
4
Diafiltration
(4)
Protein
Bm95 protein
(1)
Organism
Molecularity
MW (Da)
Solubility
Refolding Method
Redox Agent
pH
T(°C)
Boophilus microplus
Monomer
61818
insoluble
Diafiltration
None
8
20
Interferon regulatory factor 4
(1)
Organism
Molecularity
MW (Da)
Solubility
Refolding Method
Redox Agent
pH
T(°C)
Human
Unknown
51772.5
soluble
Diafiltration
None
8.5
4
Leptin
(1)
Organism
Molecularity
MW (Da)
Solubility
Refolding Method
Redox Agent
pH
T(°C)
Human
Monomer
18348.2
insoluble
Diafiltration
GSH/GSSG
7.4
4
Trypsinogen
(1)
Organism
Molecularity
MW (Da)
Solubility
Refolding Method
Redox Agent
pH
T(°C)
Human
Monomer
26398.7
insoluble
Diafiltration
GSH/GSSG
8.6
5
Dialysis
(209)
Protein
Non-structural protein 1
(1)
Organism
Molecularity
MW (Da)
Solubility
Refolding Method
Redox Agent
pH
T(°C)
Dengue virus type 2
Unknown
39939.5
insoluble
Dialysis
GSH/GSSG
0
0
1-aminocyclopropane-1-carboxylate synthase
(1)
Organism
Molecularity
MW (Da)
Solubility
Refolding Method
Redox Agent
pH
T(°C)
Cucurbita pepo (Squash)
Dimer
57033.9
insoluble
Dialysis
DTT
8
4
10 kDa chaperonin groES protein
(2)
Organism
Molecularity
MW (Da)
Solubility
Refolding Method
Redox Agent
pH
T(°C)
Escherichia coli
Unknown
10387
insoluble
Dialysis
None
7
25
Chlamydolphila pneumoniae
Heptamer
11316
insoluble
Dialysis
None
7
25
2-amino-3-ketobutyrateCoA ligase
(2)
Organism
Molecularity
MW (Da)
Solubility
Refolding Method
Redox Agent
pH
T(°C)
Thermococcus kodakarensis KOD1
Unknown
43971.5
insoluble
Dialysis
Beta-mercaptoethanol
7.5
4
Thermococcus kodakarensis KOD1
Unknown
43971.5
insoluble
Dialysis
Beta-mercaptoethanol
7.5
4
3-hydroxyanthranilic acid dioxygenase
(1)
Organism
Molecularity
MW (Da)
Solubility
Refolding Method
Redox Agent
pH
T(°C)
Human
Monomer
36352.2
insoluble
Dialysis
Beta-mercaptoethanol
8.2
4
4-aminobutyrate aminotransferase, mitochondrial also known as GABA transaminase
(1)
Organism
Molecularity
MW (Da)
Solubility
Refolding Method
Redox Agent
pH
T(°C)
Pig (Sus scrofa)
Dimer
53297.1
insoluble
Dialysis
None
7.4
4
5-hydroxytryptamine 4 receptor
(2)
Organism
Molecularity
MW (Da)
Solubility
Refolding Method
Redox Agent
pH
T(°C)
Mouse
Monomer
44883.8
insoluble
Dialysis
Beta-mercaptoethanol
7.8
25
Mouse
Monomer
44883.8
insoluble
Dialysis
Beta-mercaptoethanol
9.2
25
56kDa type-specific outer membrane antigen
(3)
Organism
Molecularity
MW (Da)
Solubility
Refolding Method
Redox Agent
pH
T(°C)
Orientia tsutsugamushi/Rickettsia tsutsugamushi
Unknown
40278.5
insoluble
Dialysis
GSSG
8
4
Orientia tsutsugamushi/Rickettsia tsutsugamushi
Unknown
40091.3
insoluble
Dialysis
GSSG
8
4
Orientia tsutsugamushi/Rickettsia tsutsugamushi
Unknown
40897.3
insoluble
Dialysis
None
8
4
60 kDa chaperonin groEL protein
(2)
Organism
Molecularity
MW (Da)
Solubility
Refolding Method
Redox Agent
pH
T(°C)
Escherichia coli
Heptamer
57197.7
insoluble
Dialysis
None
7
25
Chlamydolphila pneumoniae
14-mer
58203.9
insoluble
Dialysis
None
7
25
A33R homolog gene protein
(1)
Organism
Molecularity
MW (Da)
Solubility
Refolding Method
Redox Agent
pH
T(°C)
Ectromelia virus
Unknown
20681.4
insoluble
Dialysis
None
0
0
Acutin
(1)
Organism
Molecularity
MW (Da)
Solubility
Refolding Method
Redox Agent
pH
T(°C)
Agkistrodon acutus
Monomer
26472.9
insoluble
Dialysis
Cysteine/Cystine
7.5
25
Adenylate kinase
(1)
Organism
Molecularity
MW (Da)
Solubility
Refolding Method
Redox Agent
pH
T(°C)
Pig (Sus scrofa)
Unknown
21638.9
insoluble
Dialysis
None
7.4
25
Alanine--glyoxylate aminotransferase
(1)
Organism
Molecularity
MW (Da)
Solubility
Refolding Method
Redox Agent
pH
T(°C)
Human
Monomer
47839.2
partial
Dialysis
DTT
7
25
Alternansucrase
(1)
Organism
Molecularity
MW (Da)
Solubility
Refolding Method
Redox Agent
pH
T(°C)
Leuconostoc mesenteroides
Unknown
228850
partial
Dialysis
None
5.4
0
Amyloid beta A4 protein Cleaved into: Beta-amyloid protein 42
(1)
Organism
Molecularity
MW (Da)
Solubility
Refolding Method
Redox Agent
pH
T(°C)
Human
Unknown
13060.9
partial
Dialysis
None
8
25
Arg specific Gingipain 1
(1)
Organism
Molecularity
MW (Da)
Solubility
Refolding Method
Redox Agent
pH
T(°C)
Porphyromonas gingivalis
Monomer
57182.5
insoluble
Dialysis
DTT/cysteine
7.8
25
Aspartic proteinase Asp1
(1)
Organism
Molecularity
MW (Da)
Solubility
Refolding Method
Redox Agent
pH
T(°C)
Oryza sativa
Monomer
58761.4
insoluble
Dialysis
None
3.5
4
AtMinD1
(2)
Organism
Molecularity
MW (Da)
Solubility
Refolding Method
Redox Agent
pH
T(°C)
Arabidopsis thaliana
Monomer
35690.4
insoluble
Dialysis
DTT
7
37
Arabidopsis thaliana
Monomer
35633.3
insoluble
Dialysis
DTT
7
37
ATP synthase F1 beta subunit
(1)
Organism
Molecularity
MW (Da)
Solubility
Refolding Method
Redox Agent
pH
T(°C)
Spinacia oleracea
Monomer
53744.6
insoluble
Dialysis
DTT
8
4
Basic membrane protein A precursor
(1)
Organism
Molecularity
MW (Da)
Solubility
Refolding Method
Redox Agent
pH
T(°C)
Borrelia burgdorferi
Monomer
60452.4
insoluble
Dialysis
DTT
7.4
25
Basic membrane protein B
(1)
Organism
Molecularity
MW (Da)
Solubility
Refolding Method
Redox Agent
pH
T(°C)
Borrelia burgdorferi
Monomer
40983.9
insoluble
Dialysis
DTT
7.4
25
Basic membrane protein C
(1)
Organism
Molecularity
MW (Da)
Solubility
Refolding Method
Redox Agent
pH
T(°C)
Borrelia burgdorferi
Monomer
41227.6
insoluble
Dialysis
DTT
7.4
25
Basic membrane protein D
(1)
Organism
Molecularity
MW (Da)
Solubility
Refolding Method
Redox Agent
pH
T(°C)
Borrelia burgdorferi
Monomer
40983.9
insoluble
Dialysis
DTT
7.4
25
Beauveria bassiana Chitinase
(1)
Organism
Molecularity
MW (Da)
Solubility
Refolding Method
Redox Agent
pH
T(°C)
Beauveria bassiana
Unknown
36736.9
insoluble
Dialysis
None
8.5
25
Beta-glucosidase
(1)
Organism
Molecularity
MW (Da)
Solubility
Refolding Method
Redox Agent
pH
T(°C)
Agrobacterium tumefaciens
Unknown
88290.1
insoluble
Dialysis
None
8
4
Bikunin Placental
(1)
Organism
Molecularity
MW (Da)
Solubility
Refolding Method
Redox Agent
pH
T(°C)
Human
Monomer
25415.7
Dialysis
GSSG/DTT
8
25
Brazzein
(1)
Organism
Molecularity
MW (Da)
Solubility
Refolding Method
Redox Agent
pH
T(°C)
Pentadiplandra brazzeana
Monomer
23063.2
partial
Dialysis
None
3.9
4
Candidapepsin
(1)
Organism
Molecularity
MW (Da)
Solubility
Refolding Method
Redox Agent
pH
T(°C)
Candida tropicalis
Monomer
40559
insoluble
Dialysis
None
7
4
Canstatin
(1)
Organism
Molecularity
MW (Da)
Solubility
Refolding Method
Redox Agent
pH
T(°C)
Mouse
Unknown
149090
insoluble
Dialysis
GSH/GSSG
8.5
0
Cardiotoxin analogue III
(1)
Organism
Molecularity
MW (Da)
Solubility
Refolding Method
Redox Agent
pH
T(°C)
Taiwan cobra
Monomer
9038.2
soluble
Dialysis
None
8
25
Cathepsin B
(1)
Organism
Molecularity
MW (Da)
Solubility
Refolding Method
Redox Agent
pH
T(°C)
Human
Monomer
37821.6
insoluble
Dialysis
Cysteine
7
4
Cathepsin L1 also known as papain prosegment
(1)
Organism
Molecularity
MW (Da)