[Buckle et al (2005) Nature Methods. 2,3]    [Chow et al (2005) Protein Expr Purif. 46, 166-171] Home Graphs About RSS Feeds Help
Deposit Data   Quick Search Any Word    All Words Exact Match Table View Compare View Tree View Advanced Search Browse Database BLAST Sequence Protein Folding Database Statistics Version: 4.0 Last Updated: 2009-09-19 Refolding entries: 1157 Proteins: 759 Families: 260 Organisms: 327 View Top Ten Hits Graphical summary Latest Additions non-POU domain-containing... Interferon Beta DASH complex subunit DAD1... Outer membrane protein A... Snakin-1 Cysteine proteinase Activating transcription ... Cysteine protease falcipa... CAMP-specific cyclic nuc... Hen egg- white lysozyme... Login Username: Password: Forgot your password?  Remember Me Register an account	Home > Search > Results > Comparison Found results. Highlight: None None Similar Similar Different Different Expand All / Contract All PROTEIN Protein Name Abbreviated Name SCOP Family Structure Notes Organism UniProt Accession SCOP Unique ID Structure Solved Class Molecularity CONSTRUCT Full Length Domain Chimera Variants Chain Length Molecular Weight pI Disulphides Pfam Domain(s) and Number of Occurrences Domain Graphics Full Sequence Notes EXPRESSION Report Project Aim Fusion Protein Expression/Production Expression Host Expression Strain Expression Temperature Expression Time Expression Vector Expression Protocol Method of Induction Cell Density (at induction) Cell Disruption Method Lytic Agent Pre-refolding Purification Solubility REFOLDING Refolding Method Wash Buffer Solubilization Buffer Refolding Buffer Pre-refolding Purification Tag Cleaved Refolding pH Refolding Temperature Protein Concentration Refolding Time Redox Agent Redox Agent Concentration Refolding Protocol Assay Chaperones Additives Additives Concentration Refolding Yield Purity Notes   HIV protease  HIV-PR  Retroviral protease (retropepsin)    HIV-1  P03366  50632  y  Beta  Dimer    y  n/a  n/a  n/a  123  13420  9.28603  0  Pfam Entry RVP(1)    PQITLWQRPLV TIKIGGQLKE AL...  BLAST against Refold database  |  BLAST against NCBI NR database  n/a    Tomasseli et al., ( 1990) Biochemistry, 29, 264-269  Drug Studies...  None  Protein recombinantly expressed as and refolded from inclusion bodies.  Escherichia coli  BStl-c  37  4-5h  pURA-PR3  Cells were grown in LB me...  Temperature Shift  n/a  Sonication  None  None  insoluble    Dilution  10mM TrisHCl pH 7.4, 1mM ...  6M GdnHCl...  50mM sodium acetate pH 5....  None  no tag  7.5  4      DTT  2.5mM  Cells were harvested by centrifugation (10000g, 10...  enzyme activity...  None  None, Glycerol, Ethylene glycol  5%/10%          HIV protease  HIV-PR  Retroviral protease (retropepsin)    Human  P03366  50632  y  Beta  Dimer    y  n/a  n/a  n/a  99  10792.8  8.83  Unknown  Pfam Entry RVP(1)    PQITLWQRPLVTIKIGGQLKEALLD...  BLAST against Refold database  |  BLAST against NCBI NR database  n/a    Dash et al., ( 2005) Biochemistry, 44, 3725-3734  Folding...  None  Protein recombinantly expressed as and refolded from inclusion bodies.  Escherichia coli  MC1061  42  1 h  pPTN  Purification of HIV-1 PR ...  Temperature Shift  n/a  Sonication  None  RP-HPLC  insoluble    Dilution  100 mM NaCl, 5 mM љ...  50 mM sodium phosphate bu...  50 mM sodium phosphate bu...  RP-HPLC  no tag  6  28  n/a  6-8 h  Beta-mercaptoethanol  5 mM  Denaturation and Renaturation of HIV-1 PR. All exp...  Fluorescence...  alpha-crystallin  None  n/a  n/a  n/a      HIV protease  HIV-PR  Retroviral protease (retropepsin)    HIV  P03366  50632  y  Beta  Dimer    n  n/a  tethered dimer: protease domains tethered head-to-tail  D25G in first protease domain  273  29646.3  8.69067  0  Pfam Entry RVP(2)    MEFMEDLAFL QGKAREFSSE QTR...  BLAST against Refold database  |  BLAST against NCBI NR database  D25G mutation prevents au...    Cheng et al., ( 1990) Proc. Natl. Acad. Sci. USA, 87, 9660-9664  Functional Studies...  None  Protein recombinantly expressed as and refolded from inclusion bodies.  Escherichia coli  JM105  37  not stated  pET3  no details provided...  Not Stated  n/a  French Press  None  None  insoluble    Dialysis  n/a...  67% acetic acid...  20mM Mes, 1mM DTT, 10% gl...  None  no tag  5.5  25    2h  DTT  1mM  After expression, cells were harvested and lysed u...  Enzyme activity...  None  None, Glycerol  10%          HIV protease  HIV-PR  Retroviral protease (retropepsin)    HIV  P03366  50632  y  Beta  Dimer    n  n/a  tethered dimer: protease domains tethered head-to-tail  n/a  204  21796.8  9.13863  0  Pfam Entry RVP(2)    VTLWQRPLV TIKIGGQLKE ALLG...  BLAST against Refold database  |  BLAST against NCBI NR database  leader sequence auto-clea...    Cheng et al., ( 1990) Proc. Natl. Acad. Sci. USA, 87, 9660-9664  Functional Studies...  None  Protein recombinantly expressed as and refolded from inclusion bodies.  Escherichia coli  JM105  37  not stated  pET3  no details provided...  Not Stated  n/a  French Press  None  None  insoluble    Dialysis  n/a...  67% acetic acid...  20mM Mes, 1mM DTT, 10% gl...  None  no tag  5.5  25  n/a  2h  DTT  1mM  After expression, cells were harvested and lysed u...  Enzyme activity...  None  None, Glycerol  10%  n/a  n/a      HIV protease  HIV-PR  Retroviral protease (retropepsin)    HIV-1  P03366  50632  y  Beta  Dimer    y  n/a  n/a  n/a  113  12419.5  9.44294  0  Pfam Entry RVP(1)    MRGSHHHGSPQITLWQRPLVTIKIG...  BLAST against Refold database  |  BLAST against NCBI NR database  n/a    Leuthardt A and Roesel JL ( 1993) FEBS Letters, 326, 275-280  Drug Studies...  N-terminal and C-terminal poly-histidine tags  Protein recombinantly expressed as and refolded from inclusion bodies.  Escherichia coli  M15  37  5h  pDS  Cells were grown at 37deg...  IPTG  OD 600 = 0.7  Freeze-thaw  Lysozyme  Metal affinity chromatography  insoluble    Dialysis  50mM TrisHCl, 50mM NaCl, ...  50mM TrisHCl, 50mM NaCl, ...  20mM MES pH 6.0, 0.01% Tr...  Metal affinity chromatography  no  6  4  10-30microg/ml  24h  DTT  10mM  Cells were centrifuged and the pellet (9-10g/L) wa...  Enzyme activity...  None  None, Glycerol  29%  95%
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